Waters Hplc System Cleaning Procedure

Place a b c and d seal wash and needle wash lines into a clean.

Waters hplc system cleaning procedure.

Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa. Use solvent inlet filters to protect the system from incoming particles. This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules. Reverse osmosis to remove most contaminants b.

It is necessary to restore the system flow path to a near new level of cleanliness note. Residues or contaminations may block filters or capillaries. 2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory. To clean the system.

The purification process should include all of the following steps. When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. To clearly lay down the procedure for cleaning. When working with any lc system setting it up and storing it is very important.

Remove all solvent filters. The water flushes are only needed to remove the previous solvent from the system. Disconnect the column and attach a union and flow directly to waste. Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.

3 0 responsibility 3 1 doing. For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. Cleaning decontamination columns general guidelines flow cells general. Water flush to clear customer s mobile phases from the system.

Label bottles correctly with bottle content and filling date expiry date. Maintaining your hplc uhplc uplc systems. To clean the system. Ion exchange to remove any remaining ions.

No injections are needed with that. Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems. Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.

Responsibility quality control officer executive. 10 nh4oh in water this removes peg and other. Use only hplc grade solvents and water filtered through 0 2. Technical assistant 3 2 checking.